One shot is a combat term for a high-damage attack or hit that can kill most characters with "one shot", hence the name.

Most high-level and elite raid bosses usually have some kind of "one shot" attack. Usually you will need a strategy to avoid, debuff, or absorb these attacks via your Main Tank.

A more recent, but wrong use of the term applies to instance runs, where a party manages to finish a boss, especially a difficult one, in a single attempt.

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